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Crop researchers performing germplasm screenings are often unable to replicate their plots due to scarcity of seed and the large numbers of genotypes being evaluated. The use of known check varieties is a common method of overcoming the difficulties associated with unreplicated trials. In this simulation, we explored the effect of check plot density on the effectiveness of the resulting analysis. We also explored the effect of analyzing treatments as random versus fixed. Our study considers ten different designs with check densities ranging from 5% of the plots to 50%. The designs and analyses were then compared on the basis of the correlation of the actual treatment effects with the following: "observed" yield, LSMEANs for treatments fixed, and BLUPs for treatments random. Finally, we observed the frequency with which the analysis ranked the top 10% of the treatments within the top 15% of the LSMEANs or BLUPs. It was found that the LSMEANs and BLUPs from the spatial analysis provide more accurate results than the observed Y-values. Also, if the treatments are analyzed as fixed and the LSMEANs are used as estimates, then there seems to be a certain point beyond which not much additional information is gained by adding more check plots. This plateau is reached near a check plot density of approximately 30%. Finally, the BLUPs seem to be a more accurate estimate of the true treatment effects than are the LSMEANs at the lower densities; in fact, the BLUPs perform relatively well even at check densities of only 5% or 10%.

UNREPLICATED VARIETY TRIALS: EFFECTS OF CHECK PLOT DENSITY AND FIXED VERSUS RANDOM TREATMENTS