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Experiments have shown human chorionic gonadotropin (hCG) to be more effective than GnRH as a means to induce ovulation of follicles. Dosages used, however, have differed greatly among experiments. A study was performed to determine the minimum effective dose of hCG needed to induce ovulation of ovarian follicles in dairy cows. Ovaries of Holstein cows were mapped by using transrectal ultrasonography 7 days before a bi-weekly pregnancy diagnosis. Cows were assigned randomly to treatments of saline, 100 μg of GnRH (2 mL of Fertagyl, Intervet, Inc., Millsboro, NJ), or 500, 1000, 2000, or 3000 IU of hCG (0.5, 1, 2, or 3 mL of Chorulon, Intervet, Inc., Millsboro, NJ). Ovarian structures were monitored again 7 days later, and the proportion of cows, and proportion of follicles ≥ 8 mm in diameter, that ovulated were recorded. A dose of at least 1000 IU of hCG resulted in a greater ovulatory response than saline, GnRH, or 500 IU of hCG.

Ovulation potential of human chorionic gonadotropin versus GnRH