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Four areas of the milking parlor were evaluas colony forming units per area or volume ated for effective cleaning and sanitation using (CFU/cm or ml). The downfall of this techtotal aerobic counts (standard plate count) and nique is that it only measures the number of ATP bioluminescence (ATPB) techniques. aerobic microorganisms and not the presence of Whereas the plate counts only monitor bacterial soil or food residue. This microbial technique is numbers, the ATPB results (reported as relative time consuming (24 to 48 hr before results are light units, RLU) also indicate residual soil or available), requires a fair amount of knowledge, food residue on the surface. Results showed and is expensive (both reusable and nonreusable little correlation between the RLU values and the equipment and resources are necessary). aerobic plate count data; however, the ATP bioluminescence technique detected the presence The ATP bioluminescence (ATPB) system is of soil residue on the contact surface. The ATP relatively new. Currently, this technology is bioluminescence system is a fast (<2 min) and used to monitor sanitation effectiveness in food simple method that evaluates the effectiveness of processing plants. The ATPB monitors both cleaning and sanitation procedures employed. microbial loads and food residue but fails to

ATP bioluminescence can evaluate cleaning and sanitizing effectiveness in the milking parlor