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We evaluated the potential of several commercially available proteases for use in predicting the undegradable intake protein (UIP) concentrations o f alfalfa and prairie hay. Protease s differed in their estimates o f the rate of forage protein breakdown an d the amounts of different forage protein fractions . At least one protease appeared to yield acceptable predictions of UIP via a short-term, single time-point assay. Assays of this type deserve further consideration for commercial application.

Estimating the undegradable intake protein content of two forages by different commercial proteases