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Porcine proliferative enteropathy (PPE) caused by  (LI) is a global cause for substantial economic losses in the swine industry. Here, we constructed live attenuated  (ST) mutant strains expressing and secreting 4 selected immunogenic LI antigens, namely, optA, optB,  flagellin (LfliC), and  hemolysin (Lhly); the resultant recombinant strains were designated Sal-optA, Sal-optB, Sal-LfliC, or Sal-Lhly, respectively. Using the BALB/c mouse model, we demonstrate that mice vaccinated once orally, either with a mixture of all 4 recombinant strains or with an individual recombinant strain, show significant ( < 0.05) production of LI-specific systemic immunoglobulin (Ig) G and mucosal IgA responses compared to the  alone group. Upon restimulation of vaccinated splenocytes with the LI-specific antigens, significant ( < 0.05) and comparable production of interferon-γ responses are found in all vaccinated groups, except the Sal-Lhly group, which shows non-significant levels. Challenge studies were performed in C57BL/6 vaccinated mice. On challenge with the LI (10 50% tissue culture infectious dose) 14 days post-vaccination, 20% (1/5) of mice in all vaccinated groups, except Sal-Lhly group, show the presence of the LI-specific genomic DNA (gDNA) in stool samples. In contrast, 40% (2/5) and 60% (3/5) of mice vaccinated with the Sal-Lhly strain and the attenuated  alone, respectively, were found positive for the LI-specific gDNA. Furthermore, 0% mortality was observed in mice vaccinated against the ST challenge compared to the 30% mortality observed in the unvaccinated control group. In conclusion, we demonstrate that the -based LI-vaccines induce LI-specific humoral and cell-mediated immunities, and encompass the potential to offer dual protection against PPE and salmonellosis.

journal of veterinary science

An attenuated <i>Salmonella</i> vaccine secreting <i>Lawsonia intracellularis</i> immunogenic antigens confers dual protection against porcine proliferative enteropathy and salmonellosis in a murine model

An attenuated Salmonella vaccine secreting Lawsonia intracellularis immunogenic antigens confers dual protection against porcine proliferative enteropathy and salmonellosis in a murine model