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The present work evaluated the influence of filling substrate material (crushed PET bottles or fine gravel) on the efficiency of pollutant removal in horizontal subsurface flow constructed wetlands (HSSF-CWs). They were cultivated with a consortium of elephant grass cv. Napier ( Pennisetum purpureum Schum) and Tifton 85 ( Cynodon spp.) to treat wastewater from a common milk cooling tank (WWMT). For this, six HSSF-CWs were used which had dimensions of 0.6 m tall x 1.0 m wide x 2.5 m long. In order to investigate possible efficiency loss in the removal of pollutants from the system, operation was divided into two periods: Period I (from April to December 2015) and Period II (April to December 2016). Thus, the removal efficiencies of BOD 5 , solids and total nitrogen (TN), total phosphorus (TP), potassium (K) and sodium (Na) from WWMT were statistically compared. Results indicated that the efficiency of the HSSF-CWs for removing pollutants increased or remained similar after one year and nine months of their operation; and PET bottles were a viable alternative substrate in HSSF-CWs based on the efficient removal of pollutants from WWMT during the one year and nine months of monitoring. Crushed PET bottles constitute a viable substrate for filling HSSF-CWs. Altering the cultivation positions of the plant species did not change pollutant removal efficiencies, but indicates the importance of species arrangement to maximize system performance.

Efficiency of horizontal subsurface flow-constructed wetlands considering different support materials and the cultivation positions of plant species