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Establishment of tissue cultures derived from deep-sea multicellular organisms has been extremely difficult, because of serious damage they sustain upon decompression and exposure to high temperature of surface seawater. We developed a novel pressure-stat aquarium system and a novel pressure chamber control system for the study of living deep-sea multicellular organisms and derived cells under the pressure. Using these devices, we successfully cultivated and freeze-stocked fin cells of deep-sea eel Simenchelys parasiticus collected at 1162m under atmospheric pressure condition. [pressure-stat aquarium system, optical pressure chamber control system, deep-sea organisms, interleukin production, cytokine synthesis, tissue culture]

Molecular and Cellular Biological Studies for Deep-sea Multicellular Organisms under Atmospheric Pressure Condition