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I n d I a n J o u r n a l o f P a t h o l o g y a n d M I c r o b I o l o g y 5 9 ( 1 ) , J a n u a r y M a r c h 2 0 1 6 131 Figure 2: (a) Multiplex immunohistochemistry CD4+ CD8 cocktail: Atypical T‐lymphoid cells show strong red positivity for CD 8 and are CD4 negative (×100). (b) Immunohistochemistry ‐ CD 30 is negative (×400). (c) Polyacrylamide gel for T‐cell receptor gamma gene rearrangement. Lane 1: 100 bp ladder; Lane 2: Positive or monoclonal control (167 bp); Lane 3: Specimen control ladder (presence of all the products indicate the integrity of DNA). Lane 4: T‐cell receptor gamma MMx‐A (shows monoclonal band); Lane 5: T‐cell receptor gamma MMx‐B; Lane 6: Negative or polyclonal control c b a Figure 1: (a) Dense dermal lymphoid infiltrate and focal epidermotropism (H and E stain, ×100). (b) Dermal lymphoid aggregates (H and E stain, ×400). (c) Immunohistochemistry ‐ CD3 positive lesional cells (×100). (d) Immunohistochemistry – Ki‐67 is high index (×400) d c b a Primary cutaneous aggressive epidermotropic CD8+ cytotoxic T‐cell lymphoma: A case report and significance of timely ancillary testing

Primary cutaneous aggressive epidermotropic CD8+ cytotoxic T-cell lymphoma: A case report and significance of timely ancillary testing