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To the Editor: Male steroid hormones are synthesized in the adrenal cortex and testis, and pediatric reference intervals for normal steroid hormone levels are essential for the diagnosis of adrenal and gonadal disorders. Importantly, steroid hormone profiles vary with age and pubertal development, and to date, only very limited data have been published concerning normal steroid hormone ranges in terms of age and pubertal development. The absence of such data has been primarily due to the lack of a sufficiently sensitive and specific method for determining appropriate reference intervals for steroid concentrations.

Liquid Chromatography-Tandem Mass Spectrometry-Based Characterization of Steroid Hormone Profiles in Healthy 6 to 14-Year-Old Male Children