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Conventional and differential staining revealed that the kariotype of Eptesicus serotinus (Schreber, 1774) from the Bialowieza population consists of 24 pairs of telocentric autosomes. Only sex chromosomes have two arms; the X-chromosome is submetacentric and similar in size to that of the largest pair of autosomes, while the Y-chromosome is submetacentric and of the size of the two smallest pairs of autosomes.

Chromosomes of some species of vespertilionid bats. I. Banding patterns of Eptesicus serotinus chromosomes