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This study was performed to evaluate the effects of commercially available aromatic plant essential oil extracts (MixOilTM) on the protection outcome achieved after a Newcastle disease (ND) vaccination. Antibody production, after a MixOil treatment administered along with a vaccination program applying a live attenuated Newcastle disease virus (NDV) vaccine, was assessed under field conditions. The antibody response was evaluated via a Hemagglutination Inhibition test and an Enzyme-Linked Immunosorbent Assay. Later, the experimental groups were challenged with two velogenic NDV strains: Herts 33 and a local virulent NDV strain. A MixOil treatment effect with a higher dose was also examined. It was observed that ND antibody titers were enhanced when the birds were placed on a MixOil immune system-boosting program during vaccination. The mortality rate decreased by at least 50% after regular MixOil immune boosting; it also decreased following larger treatment doses. Clearly, promoting a strong bird immune system through herbal supplementation would naturally be expected to create a successful vaccination outcome and healthy flocks of birds. In addition, the results suggest that, by applying a higher dose of MixOil, the treatment can exceed its immune-stimulator benefits; it resulted in controlling the mortality outcome from the experimental ND infection

Boosting Newcastle disease vaccination efficacy under field conditions by aromatic plant essential oil extracts