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Background: Nigeria is one of the dengue hyper-endemic nations. This study investigated the level of knowledge about dengue and vector preventive practices and their impacts on acute dengue among febrile patients at Abuja, Nigeria. Materials and methods: This cross-sectional survey was conducted on febrile patients attending University of Abuja Teaching Hospital, Abuja, Nigeria. Blood samples were individually collected from 171 febrile patients residing at Gwagwalada suburb. Interviewer administered questionnaires were used to assess subjects’ knowledge about preventive measures against vector breeding and bites. Blood samples were tested for dengue virus Nonstructural glycoprotein-1antigen using enzyme linked Immunosorbent assay (ELISA). Results: Fifteen out of 171 febrile persons (8.8%) were Dengue NS1 positive. Sixty percent of the subjects do not know about dengue, while 33% knew about dengue virus infection through television/ radio programs, 5% through healthcare professionals and 2% from friends/families. Those who persistently use indoor residual spraying and long sleeves/trousers during daytime had less cases of DENV NS1 than those who do not. There was statistical association between DENV NS1 and residence in proximity to waste dumpsites (P<0.0001) and frequent use of long sleeve clothing and trousers (P=0.005). However, there was no statistical association between DENV NS1 antigenemia and persistent use of indoor residual spraying and presence of in-door water containers (P>0.05). Conclusions: Findings from this study imply that proper education and adherence to preventive measures minimize people from being susceptible to Dengue virus infections

Adherence to vector preventive measures decrease cases of acute Dengue among Abuja residents, Nigeria