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The presumptive laboratory diagnosis of Clostridium difficile infection is achieved by the means of the detection of a common antigen (glutamate dehydrogenase, GDH) in stool, then confirming the positives either by the detection of toxins A and B or by a molecular test for the detection of pathogenicity locus, encoding for the two toxins and for the binary toxin. A fully automated chemiluminescence system for the GDH antigen (LIAISON® C. difficile GDH) and for the detection of toxins A and B (LIAISON® C. difficile Toxin A and B) (DiaSorin, Gerenzano, Italy) allows for the performance of these tests on large numbers of samples in a short time, ensuring the traceability of the data

microbiologia medica

Evaluation of LIAISON® C. difficile glutamate dehydrogenase and LIAISON® C. difficile toxin A and B in Copan FecalSwabTM samples in a three-step algorithm for the diagnosis of Clostridium difficile infection