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. HHV7 is a CD4-positive T-lymphotropic herpesvirus, the transforming capacity of which has not been established. Few studies have investigated the possible role of HHV7 in Cutaneous T cell lymphomas (CTCL): mycosis fungoides (MF), Sezary syndrome (SS), primary cutaneous CD30+ CTCL, including Lymphomatoid papulosis (LyP). Aim of this study was to develop a real time PCR for quantification of HHV7-DNA and to investigate the role of HHV7 in the context of CTCL. Methods. To determine sensitivity of the Real-time PCR a clone containing 311 bp of the HHV7-DNA was obtained. The plasmid was then serially diluted, the concentrations were 20000, 2000, 200, 20, 2, and 0.2 copies/μl, and a 5-μl aliquot of each was used as standard. LUX primers are designed with a fluorophore near the 3 ́end in a hairpin structure. PCR was used to quantify HHV7-DNA in 191 skin biopsies of 147 pts (99M/49F, mean age 63 years): 74 MF, 37 SS, 11 CD30+ CTCL, 25 LyP. Results. The copy numbers of samples were calculated from the CT values by the software program, Sequence Detector version 1.6 (Applied Biosystems). The sensitivity of this PCR was estimated from the standard curve to be approximately 10 copies/reaction.. HHV7DNA was detected in 24 tissue samples of 20/147 (13.6%) patients: 10/37 SS, 4/11 CD30+ CTCL, 6/25 LyP and 0/74 MF. Conclusions. The LUX-Real-time assay described is highly sensitive and specific for the detection of HHV7-DNA and permits quantitative assessment of viral load. No definitive conclusion on HHV7 role in the pathogenesis of primary CTCL can be made, although it is to note that HHV7-DNA was not detected in any of the pts with MF, a less aggressive CTCL. 155

DEVELOPMENT OF A LUX-REAL TIME PCR FOR HUMAN HERPESVIRUS 7 (HHV7) IN PRIMARY CUTANEOUS T CELL LYMPHOMAS.