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Paleoceanographic surveys can be performed from the quantitative study of calcareous nannofossils since variations in the nannoflora through time indicate a biotic response to environmental changes. In this study, two preparation techniques of calcareous nannofossils slides the conventional smear slide and the random settling techniques were compared in order to investigate which method is better in quantitative studies for paleoceanographic reconstructions. The adaptation of sample preparation methods allows studies of relative and absolute abundances of calcareous nannofossils. Although the random settling technique requires a much longer preparation time, it allows the calculation of the number of coccoliths per gram of sediment. This, together with the relative abundance and stable oxygen and carbon isotopic data allowed a more detailed analysis of paleotemperature and paleoproductivity. The Gephyrocapsa genus showed good correlations with δ18O and δ13C, indicating an influence of temperature and nutrient availability on the abundance of this

Use of the random settling technique in quantitative studies of calcareous nannofossils in the NW South Atlantic Ocean