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espanolRESUMEN El almacenamiento seminal in vitro ha contribuido a la reproduccion de aves silvestres en cautiverio. Sin embargo es necesario predecir su capacidad fertilizante. Los indicadores de reaccion acrosomal (RA) espermatica se han relacionado con su capacidad fertilizante. Por lo anterior, el objetivo de este trabajo fue evaluar la capacidad de reaccion acrosomal in vitro en espermatozoides de halcon Harris, conservados en fresco y criopreservados. Se obtuvieron 64 eyaculados, los cuales fueron evaluados en fresco y posdescongelacion, 32 se diluyeron con medio Beltsville Poultry Semen Extender (BPSE) y 32 utilizando medio Lake. Se evaluaron 64 eyaculados en fresco y posdescongelacion; para la evaluacion espermatica basica se utilizo la tincion de eosina-nigrosina, y capacidad de RA fue mediante su induccion con membrana perivitelina (MPV). La movilidad espermatica en semen fresco fue mayor (P0,05). El porcentaje de espermatozoides con RA en el medio Lake, incubados con MPV posdescongelacion (45,4±2,7), fue menor (P EnglishABSTRACT Seminal storage, both fresh and cryopreserved, has contributed to the reproduction of wild birds in captivity, however, a method of predicting the fertilization capacity is needed. Indicators of the sperm acrosome reaction (AR) have been associated with its fertilization capacity. Therefore, the aim of this study was to evaluate the in vitro AR of fresh and cryopreserved Harris hawk sperm using Beltsville Poultry Semen Extender (BPSE) and Lake as diluents. 64 ejaculates were obtained and examined fresh and after thawing. The sperm basic evaluation for each ejaculate was made using eosin-nigrosin, while the ability of AR was assessed by coincubation with perivitelline layer (PVL). Sperm motility of fresh semen was higher (P0.05) after thawing. The percentage of sperm that underwent an AR when incubated with PVL post thawing in Lake (45.4±2.7) was lower (P0.05) with BPSE. It is concluded that Lake diluent was more efficient for fresh seminal storage, while BPSE diluent was more efficient for seminal cryopreservation in Harris hawk.

Evaluation of two diluents for the storage of fresh and cryopreserved semen of Harris hawk (Parabuteo unicinctus)