English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

espanolRESUMEN La quinua es un grano sagrado de origen andino cuya popularidad se debe a sus excelentes caracteristicas nutricionales y amplia plasticidad fenotipica. Es una especie vegetal que muestra una condicion mayoritaria de autogamia, con ciertos niveles de alogamia. El gen psbA presente en el cloroplasto, mayoritariamente descrito en plantas con herencia maternal, codifica para la proteina D1. Esta proteina esta relacionada con la fotosintesis, asi como tambien es un gen objetivo de distintos herbicidas, debido a que hay varias familias quimicas que inhiben la accion de la proteina D1. Basado en estos antecedentes se procedio a caracterizar el gen psbA en el cultivar de quinua Regalona-Baer. Para realizar la caracterizacion molecular, primeramente se extrajo el ADN, luego se realizo la Reaccion en Cadena de la Polimerasa (PCR), utilizando la informacion de las bases de datos de especies relacionadas. Los resultados muestran que el gen psbA en la quinua tiene una simple copia, con un total de 1.062 nucleotidos, generando 354 aminoacidos incluyendo el codon de "parada". El tamano total de la secuencia que codifica el gen psbA mostro un tamano similar a las 300 secuencias de genes psbA existentes en "La base de datos genoma del cloroplasto". El gen psbA podria ser el comienzo para explorar la diversidad en el genoma del cloroplasto de la quinua, conocer el tipo de segregacion extracromosomal y sobre todo el potencial de poder generar cultivares con resistencia a herbicidas asociados a este gen. EnglishABSTRACT Quinoa is a sacred grain of Andean origin whosepopularity is due to its excellent nutritional characteristics and broadphenotypic plasticity. It is a plant species that shows a majority condition of autogamy, with certain levels of alogamy. The psbA gene present in the chloroplast, mostly described in plants with maternal inheritance, codes for D1 protein. This protein is related to photo-synthesis, as well as a target gene of different herbicides, since there are several chemical families that inhibit the action of D1 protein. Based on this background, we proceeded to characterize the psbA gene in the Regalona-Baer quinoa cultivar. To perform the molecular characterization, the DNA was first extracted, and then the Polymerase Chain Reaction (PCR) was performed, using information from the related species databases. The results show that the psbA gene in quinoa has a single copy, with a total of 1062 nucleotides, generating 354 amino acids including the "stop" codon. The total size of the sequence encoding the psbA gene showed a similar size to the 300 sequences of psbA genes existing in "The Chloroplast Genome Database". The psbA gene could be the beginning to explore the diversity in the chloroplast genome of quinoa, to know the type of extra-chromosomal segregation and, above all, the potential to be able to generate herbicide-resistant cultivars associated with this gene.

Caracterización molecular del gen psbA en Chenopodium quinoa Willd