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The etiolation and layering technique that is used on avocados has two critical steps, namely, the rooting of clonal rootstock and graft union formation between the commercial variety and clonal rootstock. This study analyzed the rooting step of the clonal rootstock regarding its influence on graft union formation and development. The optimum times for grafting the commercial variety onto clonal avocado rootstock were determined under two greenhouse conditions, heating and no heating. The results showed that under greenhouse conditions without heating, there was higher survival (%) in plants that were grafted 60 days after the rooting steps in the clonal scion, and, in the experiments in a heated greenhouse, the highest survival rate (%) was observed in plants that were grafted 15 days after the rooting treatments. For both assays, the lowest survival rate was observed for grafts performed 30 days after the wounding and auxin application process. In histological cross sections of rootstocks at the wounding zone and in auxin treatments, a large number of emerging adventitious roots were developed at 30 days. La tecnica de etiolacion y acodo utilizada en palto presenta dos etapas criticas: el enraizamiento del portainjerto clonal y el prendimiento del injerto entre la variedad comercial y el portainjerto clonal. En este estudio se evaluo la influencia de la fase de enraizamiento del portainjerto clonal sobre el prendimiento y desarrollo del injerto. Definiendo los momentos optimos para realizar la injertacion de la variedad comercial sobre el portainjerto clonal de palto, en dos condiciones de invernadero con y sin calefaccion. Los resultados mostraron para las condiciones de invernadero sin calefaccion, mayor prendimiento (%) en plantas injertadas a los 60 dias despues de los pasos de enraizamiento en la pua clonal, mientras que en el ensayo realizado en invernadero calefaccionado el mayor prendimiento (%) se observo en plantas injertadas a los 15 dias despues de realizados los tratamientos de enraizamiento. Para ambos ensayos, el menor prendimiento (%) se observo en plantas injertadas a los 30 dias despues de realizado el lesionado y la aplicacion de auxinas. En cortes histologicos realizados al portainjerto, en la zona del lesionado y aplicacion de la mezcla auxinica, a los 30 dias, se observo gran cantidad de raices adventicias emergiendo.

CLONAL PROPAGATION OF THE AVOCADO: EFFECTS OF THE ROOTING STEP ON GRAFT UNION FORMATION AND DEVELOPMENT