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The effect of inoculum size on the axenic in vitro production of  Steinernema carpocapsae , using a transparent medium with hemoglobin and cholesterol, was studied. Axenic cultivation was performed with fertile eggs of S . carpocapsae (hatching of eggs: 89 ± 4%), which were obtained by lyses of female nematodes and subsequent incubation. Two important aspects of this work include the use of a completely transparent medium enriched with hemoglobin and cholesterol and the high multiplication factor of the population (MFIP) of 87, which is considered high for in vitro axenic cultures of nematodes. In vivo methods have the advantage of high MFIP, but they also have the disadvantage of the high number and complexity of the operations involved for the purposes of harvesting axenic nematodes. It is assumed that the availability of a high number of nematodes in a transparent medium will further encourage the characterization of new native strains of nematodes. Se estudio el efecto del tamano de inoculo en la produccion axenica in vitro de Steinernema carpocapsae , utilizando un medio de cultivo transparente enriquecido con hemoglobina y colesterol. El cultivo axenico se llevo a cabo con huevos fertiles de S. carpocapsae (incubacion de los huevos: 89 ± 3%) obtenidos por lisis de nematodos hembras y su subsecuente incubacion en medio de cultivo adicionado con antibioticos. Dos aspectos importantes a destacar son el uso de un medio de cultivo totalmente transparente enriquecido con la hemoglobina y colesterol, y el alto factor de multiplicacion de la poblacion (MFIP) de 87, que se considera alto para cultivos axenicos in vitro . Los metodos in vivo tienen la ventaja de su alta MFIP, pero tambien tienen la desventaja del elevado numero y complejidad de las operaciones involucradas en el proceso de cosecha de nematodos. Se considera que la disponibilidad de un alto numero de nematodos en un medio de cultivo transparente favorecera la caracterizacion de cepas nativas nuevas de nematodos.

Effect of the inoculum size on the axenic in vitro production of Steinernema carpocapsae