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espanolSe evaluo el efecto de temperatura y concentracion del hidrolizante en la extraccion de colageno soluble en acido a partir de tres regiones anatomicas de la tilapia roja (Oreochromis spp): escamas, espinas y pieles. Se optimizo el proceso de extraccion aplicando dos disenos experimentales en cada matriz estudiada en las etapas de hidrolisis basica (NaOH) para la liberacion de proteinas del tejido conectivo (PTC) e hidrolisis acida (CH3-COOH) para la obtencion del colageno. Los resultados revelaron que a 25°C y 0.4M de NaOH en la hidrolisis basica, se obtiene la mayor liberacion de PTC en las matrices estudiadas. Ademas, se puede alcanzar el maximo porcentaje de colageno (% Cl) en la hidrolisis acida con una solucion 0,7M de CH3-COOH y temperaturas de 18,5°C, 11,0°C y 21,5°C para piel (0,88%), escamas (2,16%) y espinas (0,51%), respectivamente. EnglishThe effect of temperature and concentration of the hydrolyzing agent on the extraction of acid soluble collagen from three anatomical regions of red tilapia (Oreochromis spp) was evaluated: scales, bones and skin. The extraction process is optimized using two experimental designs in each matrix studied in the stages of basic hydrolysis (NaOH) for the release of connective tissue proteins (CTP) and acid hydrolysis (CH3 COOH) to obtain collagen. Results showed that at 25°C and 0.4M solution of NaOH in the basic hydrolysis the maximum CTP in the studied matrixes are obtained. Also, the optimum condition of collagen extraction by acid hydrolysis can be achieved at 0.7M solution of CH3-COOH and temperatures of 18.5°C, 11.0 °C and 21.5°C for skin (0.88%), scales (2.16%) and spines (0.51%), respectively.

información tecnológica

Optimización de la Extracción del Colágeno Soluble en Ácido de Subproductos de Tilapia Roja (Oreochromis spp) mediante un Diseño de Superficie de Respuesta