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The chemical composition of aristolochic acids (AAs) from the roots of A. chilensis was determined by high-performance liquid chromatography with diode-array detection (HPLC-DAD), a technique widely used for the detection and quantification of AAs in herbal medicines. The roots contained a mixture of AA-I (1), AA-II (2), AA-III (3), AA-IV (4), AA-Ia (5), AA-IIIa (6), AA-IVa (7), and aristoloside (8), indicating that A. chilensis is not suitable for use as a medicinal plant due to the harmful effects of the aristolochic acids. INTRODUCTION Species of the genus Aristolochia (Aristolochiaceae) have been used in folk medicine throughout the world to treat various diseases 1 . Aristolochia can be characterized by their levels of aristolochic acids (AAs), a group of 10-nitrophenanthrene-1-carboxylic acids that normally include a 3,4-methylenedioxy moiety and substitutions at C-6 and/or C-8 with –OCH 3 filtrate was washed with CHCland –OH groups; AAs with the former substitution are known as phenolic AAs. Structures with other types of substituents have also been identified, including N-glycosides and O-glycosides of phenolic AAs.

ARISTOLOCHIC ACIDS IN THE ROOTS OF ARISTOLOCHIA CHILENSIS, A DANGEROUS CHILEAN MEDICINAL PLANT