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The RP-HPLC analytical method with UV detection at 230nm with ODS Hypersil C 18 column (250mm x 4.6mm, 5µ particle size) was developed for simultaneous determination of Adriamycin, Bleomycin, Vinblastine and Dacarbazine in plasma of lymphoma patients using mobile phase composition of 300 volumes Acetonitrile and 700 volumes 0.05M Disodium Hydrogen Phosphate containing 0.5ml TEA and pH of the mobiles phase was maintained at 3.7 with 2M Phosphoric acid at a flow rate of 0.75ml/minute with linearity ranges of 0.05-50, 0.06-50, 0.075-50 and 0.09-50µg/ml respectively with LOD and LOQ of 0.020, 0.050; 0.045, 0.060; 0.060, 0.075 and 0.040, 0.090 respectively. % recovery of DOX, BLM, VBL and DTIC were 99.24, 99.23, 99.08 and 99.09 respectively. Key Words: HPLC, DOX, Bleomycin, Vinblastine, Dacarbazine, Hodgkin’s Lymphoma. INTRODUCTION Lymphoma is a type of cancer which causes extramedullary tumors arising primarily in the lymph nodes or other sites, these tumors are called lymphomas. There are two types of Lymphomas; Hodgkin’s disease (HD) and Non-Hodgkin’s disease (NHL)

journal of the chilean chemical society

"RAPID AND SIMULTANEOUS DETERMINATION OF ADRIAMYCIN, BLEOMYCIN, VINBLASTINE AND DACARBAZINE IN PLASMA OF HODGKIN'S LYMPHOMA PATIENTS BY A REVERSED PHASE HPLC METHOD"