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Thirty years ago, I discovered a new enzyme, glycoamidase A that cleaves carbohydrate moiety from glycopeptides without aŠecting peptide structures. This brought me to determination that I should commit comprehensive analysis of N-linked oligosaccharide structures in glycoproteins by using this enzyme. Now, the HPLC mapping method developed by myself is a powerful tool to identify the structures of N-glycans. Here I described my 30 years in the dream of the structural analyses of the Nlinked oligosaccharides from the discovery of glycoamidase A to the development of the GALAXY database. A. Introduction In 1977, when I was working as an associate Professor in the Medical School of Nagoya City University, I discovered a new enzyme that cleaves the intact Nlinked carbohydrate moiety from a glycoprotein without aŠecting the carbohydrate or peptide structures, and I named this enzyme as glycoamidase A (1–3). I discovered one such enzyme, which was a previously undetected byproduct of a crude almond-derived bglucosidase preparation supplied by the Sigma-Aldrich Corporation. The International Enzyme Committee named the enzyme after its discoverer (Noriko Takahashi) and gave it an independent new number, EC. 3.5.1.52. Since then, I have been conducting comprehensive analyses of the N-linked oligosaccharide structures in glycoproteins by using this enzyme. Fortunately, it was shortly after her professor had suddenly moved out of the school and she had the opportunity to carry on this project. At that time, the author had been working on determination of the primary amino acid sequence of Stem

trends in glycoscience and glycotechnology

My 30 Years in the Dream of the structural Analyses of the N-linked Oligosaccharides: The Discovery of Glycoamidase A to the Development of the GALAXY Database