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We have applied bacteriophage display technology to construct and analyze the diversity of an IgG library of &gt;1 x 108 clones from an adult sheep immunized against the hapten atrazine. We have identified eight new VH gene families (VH2-VH9) and five new Vkappa gene families (VkappaV-VkappaIX). The heavy and kappa light chain variable region gene loci were found to be far more diverse than previously thought.

Analysis of the Diversity of a Sheep Antibody Repertoire as Revealed from a Bacteriophage Display Library