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Burkholderiapseudomallei is the causative agent of the severe community-acquired infectious disease called melioidosis. This bacterium is a Gram-negative saprophytic bacillus that resides primarily in soil and water. The indirect hemagglutination assay (IHA) has found widespread use for sero-surveillance in areas endemic for this disease and has a limited use in the diagnosis of acute melioidosis. However, standardized reagents for the IHA are not available commercially and the test has to be established in-house. The purpose of this study was to extract antigens from different B. pseudomallei strains of Sri Lankan origin, determine the optimal dilution of each strain in the IHA test and create suitable combinations of strains for use in our laboratory.

sri lankan journal of infraction diseases

Determination of optimum dilutions of extracted antigens from &lt;em&gt;Burkholderiapseudomallei&lt;/em&gt; strains of Sri Lankan origin for the diagnosis of melioidosis using the indirect hemagglutination assay (IHA)