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Purpose: In the field of nutrition, probiotics are widely in use and lactic acid from probiotics acts as the best nourishing agent. Production of lactic acid from probiotics requires a large quantity of De Man, Rogosa and Sharpe agar (MRS) media that doubles the cost of production and may also affect vegan choice. So there arises a need to develop a cost-effective new media that replaces the animal sources. This study is about the optimizing and improving a costeffective growth medium by replacing animal products with high nutritional cheap materials obtained from plants to produce lactic acid by isolated Lactobacilli strains from pure sources.  Method: This work is a comparative analytical study following use of different media for determination of best carbon sources (dates, pineapple, molasses, sucrose, fructose, galactose) and nitrogen sources (peanut, chickpea, wheat, lentils, Bengal gram, mung beans) among the different sources available to constitute the best media which is cost effective for the production of lactic acid. Results: In the optimization of nitrogen source, the highest growth was observed with the lentil seed powder. In the optimization of the carbon source, the highest increase was observed with molasses. Molasses a by-product of the sugar industry, incorporated as a carbon source, lentils as the nitrogen source in the media showed best results in terms of lactic acid production. Conclusion: The low-cost media is obtained by using plant sources, making it suitable for vegan choice. Animal products can be replaced with plant sources for the costeffective production of probiotic lactobacilli and lactic acid.

Isolation, optimization and estimation of probiotic <em>Lactobacillus</em> from different natural sources