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T h ~ s tudy was carried out to regenerate haploids from cultured anthers of t e a clones. Morphological and histological studies on t h e an ther callus dcvclopmcnt revealed t h a t nuclei of' numerous microsporcs hegan to divide unerjually, forming multicellular s t~ucturcs during the first week of culture and anther lohe.; swelled gradually until hursting. The rate of callus induction was rapid during 6-10 wccks and compact greenish calli were formed from anthers . Calli llecamc more heterogeneous wit11 time in culture. The determination of ploidy levels in anther callus showed that two levels ofploidy were present in callus. In the callus, the pei~entage of haploid cells was more (68%) than that of diploid (6%). The study on cornpailson of callus growth in antllel-s of different clones indicated that the sulvival of anthers of three clones TRI 2043, TRI 2023 and TRI 2025 was high (Iigllcst was 98%, the lowest 78%) and calli were produced in anthers of all clones used in this tiial. TRI 2043 exhibited relatively more callus formation (76.2 mg) from an ther cultured in half Murashige and Skoog (MS) medium with 2,4 D and 13AP grown in light, followed by TRI 2023, TRI 2024, TRI 2025 and lTI777. In the dark, significant callus growth was ohsewed in four clones (TRI 2025, TRI 2024, TRI 2023 and TRI 777) . Calli that formed in light turned dark green, merislcmoidlike stiuctures after transferto the same medium without 2,4 D. However, plantlets could not he regenerated.

CALLUS FORMATION IN ANTHER CULTURE OF TEA CLONES, CAMELLIA SINENSIS (L.) KUNTZE