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Water soluble and biocompatible 3-mercaptopropionic acid capped gallium selenide nanocrystals, were synthesized from hydrated gallium (III) perchlorate and selenide ions. The 3-mercaptopropionic acid capped gallium selenide nanocrystals, was non-fluorescent but showed a sharp UV-vis absorption maximum at 250 nm.The synthesized nanoparticle was used to develop an electrochemical biosensor for the detection of 17-alpha-ethinyl estradiol, an estrogenic endocrine disrupting compound (e-EDC). The biosensor was fabricated by potentiostatic deposition of novel gallium selenide nanocrystals on a L-cystine modified gold electrode, followed by covalent coupling of genetically engineered cytochrome P450-3A4 (CYP3A4), a Heme containing enzyme. The biosensor gave an electrochemical response at about-220 mV. The results revealed that 3-Mecarptopropanoic acid capped Gallium Selenide nanocrystals can be used in conjunction with CYP3A4 as an electrode modifier for the detection of 17-alpha ethinyl estradiol. The 3-Mecarptopropanoic acid capped Gallium Selenide nanoparticles exhibited a semiconductor like behaviour.

nano hybrids

3-Mercaptopropionic Acid Capped Ga&lt;sub&gt;2&lt;/sub&gt;Se&lt;sub&gt;3&lt;/sub&gt;nanocrystal-CYP3A4 Biosensor for the Determination of 17-Alpha-Ethinyl Estradiol in Water