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The main factors affecting ultrasonic extraction, such as extraction solvent, extraction temperature and extraction time were optimized for benzoic acid determination by using UV-Visible spectrophotometer at a wavelength of 230 nm. Under the optimum conditions of ultrasonic extraction, linearity between the concentration of benzoic acid in spiked curry paste samples and the absorbance was obtained in the concentration range from 100 to 2,000 mg/kg. Recoveries for benzoic acid determination were obtained range from 74.67 to 95.33%, 71.78 to 97.56% and 88.00 to 106.45% with R.S.D. were 1.05 to 5.47%, 0.92 to 4.27% and 0.71 to 4.22%, when spiked red curry paste, southern red curry paste and southern sour curry paste samples were analyzed, respectively.

The Optimization of Ultrasonic Extraction for Benzoic Acid Determination in Curry Paste Samples by Using UV-Visible Spectrophotometry