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The objective of this research was to isolate bacteria able to utilize glycerol as carbon source and study the optimal condition of 1,3-propanediol production from crude glycerol. The bacteria B-2, B-4 and B-7 could grow and produce 1,3-propanediol in basal medium formula 1,2 and 3 that containing glycerol as energy and carbon sources. The bacteria B-2 produced highest 1,3-propanediol in basal medium formula 2. In addition, the various factors that influenced to crude glycerol fermentation in basal medium formula 2 by the bacteria B-2 were investigated. It was found that the optimal conditions of 1,3-propanediol production were 10% bacterial inoculums and crude glycerol about 50 g/L was added. pH of basal medium was adjusted around 7 and the bacterial culture was incubated at 30°C with shaking at 100 rpm for 3 days. The highest yield of 1,3-propanediol was obtained about 0.4167 g/L.

Isolation of Bacteria from Clarifier Effluent of Sour Pork Industry Able to Produce 1,3-Propanediol from Crude Glycerol