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O estudo avaliou a metodologia do substrato difusor de nutrientes em condições controladas de laboratório (20oC), visando posterior aplicação em ensaios de enriquecimento in situ. A unidade experimental constou de recipiente (copo de poliestireno) preenchido com solução agar 2% e com adição isolada ou combinada de nitrogênio (0,75 M NaNO3) e fósforo (0,05 M KH2PO4). A abertura do recipiente foi revestida com malha de plâncton (20 µm) como substrato para o perifíton. As concentrações diárias de difusão dos sais foram determinadas durante 33 dias. Os coeficientes gerados mediante regressão linear foram iguais nos diferentes tratamentos (0,05dia-1). Conclui-se que as difusões dos sais não foram influenciadas pela adição combinada ou isolada de nutrientes permitindo a comparação entre os tratamentos. A partir do 4º dia, as razões N:P de difusão foram mantidas próximas da pré-estabelecida. A metodologia proposta tem uso potencial na avaliação da limitação por nutrientes em sistemas aquáticos tropicaisThis study evaluated the nutrient-diffusing substrate method in laboratory under controlled temperature (20oC), for further application in in situ bioassay enrichment experiments. The experimental unit comprised a 300mL polystyrene pot filled up with 2% agar solution with isolated or combined addition of nitrogen (0.75 M NaNO3) and phosphorus (0.05 M KH2PO4). The topside opening of the pot was covered with plankton net cloth used for periphyton growth. Nutrients daily release rates were determined during 33 days. Diffusion coefficients obtained by regression analysis were similar in all treatments (0.05day-1). Results indicated that nutrient diffusion was not influenced by isolated or combined addition, thus allowing comparison between the treatments. In addition, after 4 days N:P diffusing ratio was kept close to the previously established condition. The proposed method has a potential use for nutrient limitation assessments in tropical aquatic ecosystem

Substrato difusor de nutrientes (SDN): avaliação do método em laboratório para experimentos in situ com perifíton