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Current paper assesses the relationship between in situ incubation time, particle size and types of materials on estimates of indigestible fractions of dry matter (iDM) and neutral detergent fiber (iNDF) in cattle feed and feces. Samples of soybean meal, alfalfa and feces of cattle fed on high concentrated diets were analyzed. The samples were processed in a Wiley mill, with 0.5 and 1.5 mm porosity for food and 1.5 mm for feces, which were packed in 4 × 5 cm F57 bags (Ankon®) and nonwoven fabric (100 g m-2) of two brands. The material was divided into two groups, with and without treatment with acetone, and five replications. Samples of each group were incubated in the rumen of a multiparous cow adapted to a 70:30 diet, forage:concentrate diet, for 240 hours. The iDM and iNDF levels were evaluated sequentially to interpret the percentages of undigested material. Data underwent analysis of variance (ANOVA, p < 0.05) and means were compared by Tukey’s test (p < 0.05). Material, size of particles and incubated material affected iDM and iNDF estimates

<b>Estimates of <i>in situ</i> digestibility and fibrous compounds in feeds for ruminants