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The objective of the present paper was to establish a protocol for in vitro multiplication of the Philippine wax flower. Axillary shoots were inoculated in MS medium, holding the following concentrations of plant growth regulators during the isolation stage: without plant growth regulator; benzylaminopurine(BAP) 2.25 mg L-1; BAP 2.25 mg L-1 + naphthaleneacetic acid (NAA) 0.93 mg L-1; BAP 2.25 mg L-1 + indoleacetic acid (IAA) 0.87 mg L-1; BAP 4.95 mg L-1; BAP 4.95 mg L-1 + NAA 0.93 mg L-1; BAP 4.95 mg L-1 + IAA 0.87 mg L-1  and shootings from this stage were cultivated in a new MS medium following different combinations of plant growth regulators: without plant growth regulator; BAP 2.25 mg L-1; BAP 3.37 mg L-1; BAP 4.50 mg L-1; BAP 2.25 mg L-1 + NAA 1.12 mg L-1; BAP 3.37 mg L-1 + NAA 2.25 mg L-1; BAP 4.50 mg L-1 + NAA 3.37 mg L-1 and shooting multiplication rate per replicate was evaluated. The best treatment, during the isolation stage, was BAP 4.95 mg L-1 + IAA 0.87 mg L-1. During the multiplication stage, there were three to four shootings per replicate, regardless of the concentrations and combinations of plant growth regulators used. The seedlings rooted, forming clusters on MS medium without plants growth regulators, resulting in eight to ten seedlings per cluster.O objetivo deste trabalho foi estabelecer um protocolo para a multiplicação in vitro de Bastão-do-imperador (Etlingera elatior). Brotos axilares foram inoculados em meio MS contendo as seguintes concentrações de reguladores de crescimento durante a fase de isolamento: testemunha; BAP 2,25 mg L-1; BAP 2,25 mg L-1 + ANA 0,93 mgL-1; BAP 2,25 mg L-1 + AIA 0,87 mg L-1; BAP 4,95 mg L-1; BAP 4,95 mg L-1 + ANA 0,93 mg L-1; BAP 4,95 mg L-1 + AIA 0,87 mg L-1. As brotações provenientes desta fase foram subcultivadas em um novo meio de cultura MS: testemunha; BAP 2,25 mg L-1; BAP 3,37 mg L-1; BAP 4,50 mg L-1; BAP 2,25 mg L-1 + ANA 1,12 mg L-1; BAP 3,37 mg L-1 + ANA 2,25 mg L-1; BAP 4,50 mg L-1 + ANA 3,37 mg L-1 e avaliou-se a taxa de multiplicação dos brotos por repetição. Durante a fase de isolamento, o melhor tratamento foi BAP 4,95 mg L-1+ AIA 0,87 mg L-1. Durante a fase de multiplicação, ocorreram em média três a quatro brotações por repetição, independentemente das concentrações e combinações de reguladores de crescimento utilizadas. As plântulas enraizaram e formaram touceiras no meio MS sem reguladores de crescimento, resultando em oito a dez plântulas por touceira

Estabelecimento de protocolo para multiplicação in vitro de Bastão-do-imperador (Etlingera elatior) Jack RM Sm.