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A new methodology of inoculating Uromyces appendiculatus in tests aimed at evaluation of common bean resistance to rust is proposed. The common bean lines Golden Gate Wax (gene Ur-6) and Ouro Negro (gene Ur-ON) were crossed to obtain a F2 population, which was inoculated with race 29-3 of U. appendiculatus. Race 29-3 is incompatible with ‘Ouro Negro’ and compatible with ‘Golden Gate Wax’. Primary leaves of ten-days-old F2 plants were detached and inoculated by immersion into an inoculum suspension. Immediately following immersion, each F2 leaf was placed in a Petri dish containing a filter paper previously moistened with distilled water. These dishes were incubated in a BOD at 20°C, under a 12h daily light regime. The humidity in the dishes was maintained by addiction of 1.5 mL of distilled water in filter paper, under a three days regime. As control, the remaining primary leaves of ten-days-old F2 plants were inoculated using the conventional inoculation method. Both inoculation methods were efficient in evaluating the reaction of the common bean to U. appendiculatus. The resistance/susceptibility screening of the F2 population confirmed the monogenic dominant nature of the rust resistance controlled by the Ur-ON gene in both methods.É proposta uma nova metodologia para inoculação de Uromyces appendiculatus a fim de avaliar a resistência do feijoeiro à ferrugem. As linhagens Golden Gate Wax (gene Ur-6) e Ouro Negro (gene Ur-ON) foram cruzadas para a obtenção de uma população F2, que foi inoculada com a raça 29-3 de U. appendiculatus. Essa raça é incompatível com Ouro Negro e compatível com Golden Gate Wax. Folhas primárias das plantas foram destacadas dez dias após a semeadura e inoculadas por submersão em suspensão de inóculo. Posteriormente, cada folha foi transferida para uma placa de Petri contendo papel de filtro umedecido com água destilada, e as placas foram, então, incubadas em BOD a 20°C, em regime de 12h de luz dia-1. A umidade no interior das placas foi mantida pela adição de 1,5 mL de água destilada em intervalos de três dias. Como controle, as folhas primárias remanescentes das plantas F2, no mesmo estágio de desenvolvimento, foram inoculadas utilizando o método convencional de inoculação. Ambos os procedimentos de inoculação foram eficientes para avaliar a reação do feijoeiro ao U. appendiculatus. Em ambos os métodos, a análise fenotípica da população F2 confirmou a natureza monogênica dominante da resistência à ferrugem conferida pelo gene Ur-ON

Teste de resistência ao Uromyces appendiculatus por meio da inoculação do patógeno em folhas destacadas do feijoeiro