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Twenty lettuce accessions from the UENF Germplasm Collection were assessed for genetic diversity using RAPD markers. Multivariate statistical techniques were used, such as grouping analyses, using the Tocher and Single Linkage methods. Analysis of 55 polymorphic markers, obtained with 25 primers, showed that there was sufficient variability in the material studied to be exploited in future breeding programs. The ‘BGH 4060’ and ‘Grand Rapids’ accessions were the most dissimilar, while ‘BGH 4325’ and ‘BGH 4326' were the most similar. There was high agreement between ecogeographic origin and molecular similarity. It was further found by the stress statistic (Kruskal, 1964) and by the correlation between number of markers and increase in ideal formation of groups that 50 polymorphic markers is the optimum number for genetic diversity study in the assessed accessions.

Variabilidade genética em alface (Lactuca sativa L.) e determinação do número ótimo de marcas RAPD para estudos de diversidade molecular