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Differentiation from formae specials and races of Fusarium oxysporum by morphological and cultural characteristics is difficult and uncertain. Random amplified polymorphic DNA (RAPD) markers and ITS1 and ITS2 (internal transcribed spacer) sequences were used to evaluate genetic variability among four different formae specials of Fusarium oxysporum (F. o. cubense, F. o. lycopersici, F. o. phaseoli and F. o. vasinfectum) and two races (1 and 2) of  F. o. lycopersici. Genetic variability up to 50% was observed among the formae specials and 7% between races 1 and 2 of F. o. lycopersici using RAPD molecular markers. Similarity more than 97.2% among  formae specials and races 1 and 2 of F. o. lycopersici was observed at ITS1 and ITS2 regions, whereas the 5.8S rDNA gene was 100% homologous among isolatesDiferenciação de formae speciales e raças de Fusarium oxysporum através de características morfológicas e culturais é bastante difícil e muitas vezes duvidosa. Marcadores moleculares RAPD e sequenciamento das regiões ITS1 e ITS2 (internal transcribed spacer) e o gene 5.8S rDNA foram utilizados para estimar a variabilidade genética existente entre quatro formae speciales de Fusarium oxysporum (F. o. cubense, F. o. lycopersici, F. o. phaseoli and F. o. vasinfectum) e entre 2 raças de F. o. lycopersici. A variabilidade genética em nível de DNA genômico utilizando-se marcadores moleculares RAPD foi de até 50% entre as formae speciales e, entre as raças 1 e 2 de F. o. lycopersici foi de 7%. Houve similaridade maior que 97,2% entre as diferentes formae speciales e raças fisiológicas de F. o lycopersici na regiões correspondentes a ITS1 e ITS2, enquanto que o gene 5.8S rDNA apresentou 100% de homologia entre os isolado

acta scientiarum. agronomy

Variabilidade genética entre &lt;em&gt;formae speciales&lt;/em&gt; de &lt;em&gt;Fusarium oxysporum&lt;/em&gt; e raças 1 e 2 de &lt;em&gt;F. oxysporum&lt;/em&gt; f. sp. &lt;em&gt;lycopersici&lt;/em&gt; através de RAPD e sequências de regiões ITS e rDNA