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of calorimetric to effective (van™t Hoff) enthalpies gives the value 0.83 ±0.1 whichis fairly close to 1.0, indicating that the denaturation process of insulin proceedsas usually observed for compact globular proteins. The melting process of a me-dicinal preparation of Zn(II)-insulin, which is not an optically transparent solu-tion, has three stages with

Microcalorimetric studies of insulin and Zn(II)-insulin over a wide range of pH and protein concentration