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Hydroxyapatite particles (HAp) have been widely used by many dental implant systems as an implant coating material because of their osteoconductive properties. This study aimed at improving the antibacterial effect of HAp as a substitute for antibiotic agents which can increase drug resistance. HAp/gray titania was selected as the coating material for on the titanium alloy substrate due to its antibacterial properties after photocatalytic reaction. When combined with amino acids, HAp can form a fluorescent complex which enhances this property. Before clinical application, this new coating should be examined for cytotoxic effects against biological cells or tissues. Therefore, L929 mouse fibroblasts were used to represent fibrous tissue surrounding dental implant. After performing a 6-day alamarBlue assay, the new coating method using hydroxyapatite-tyrosine complex with gray titania on titanium alloy surface can be said to have no influence on the growth of fibroblasts.

Cytotoxicity of hydroxyapatite-tyrosine complex with gray titania coating on titanium alloy surface to L929 mouse fibroblasts