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In the metabolism of drugs, as in chemical substances, acetylation is one example of the most common pattern of genetic polymorphism. We established an easy, rapid and specific method of distinguishing the genetic polymorphism of N-acetyltransferase, which is an application of the PCR-RFLP method, and is designed to be used in the clinical setting.This method can extract deoxyribonucleic acid from a mouth mucous membrane sample, without the need to use an analysis kit or a blood sample. It was possible to clearly distinguish 4 alleles of N-acetyltransferase.

Rapid Genotyping Method of N-acetyltransferase Polymorphism Analysis Using PCR-RFLP in Human Buccal Mucous Membrane.