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The 1,3-dipolar cycloaddition of 3-methylquinoxaline-2-thione and their N-alkylated derivatives to diphenylnitrile imine is presented. Using this method, spiro[thiadiazoline-quinoxaline] derivatives as biologically interesting compounds were produced in high to excellent yields. These compounds have been characterized on the basis of their spectroscopic et spectrometric data (H and C NMR, IR, MS and X-ray). Antibacterial activity of the synthesized products has been studied by employing five bacterial strains. The compounds with ethyl group showed the best activity with MIC value of 32 μg/mL against Streptococcus fasciens.

Synthesis and antibacterial activity of new spiro[thiadiazoline-quinoxaline] derivatives