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The polycondensation of N-(O,O-di-n-hexadecyl)phophorylalanine 1 at the air/water interface has been studied. It is found that it can form stable insoluble monolayer (Langmuir film) at the air/water interface. Among the materials collected from the air/water interface, dipeptide derivative was detected by electrospray ionization mass spectrometry (ESI-MS), and the structure was further identified by tandem mass spectrometry (MS/MS), which suggested that there formed the peptide without any coupling reagents. The transmission FT-IR spectra of the subsequent Langmuir-Blogget film (LB film) confirmed the polycondensation of 1 at the air/water interface.

Polycondensation of N-(O,O-di-n-hexadecyl)phophorylalanine at the air/water interface