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The tocopherol contents of refined soybean oil with the addition of rosemary, oregano, garlic, annatto seeds and TBHQ was evaluated during storage at 25 °C and 35 °C for twelve months, in comparison with a control soybean oil without the antioxidant addition. The method proposed to assess the tocopherol content was validated and the uncertainty estimation was determined. The method presented adequate linearity and precision, accuracy between 93% and 103% and expanded uncertainty of 2%. The contents of α-, γ- and δ-tocopherols of all the tested soybean oils remained constant during the storage at 25 °C and 35 °C regardless of antioxidant addition, while β-tocopherol content decreased. The addition of a mixture of rosemary, oregano, garlic and annatto seeds increased the concentration of γ- and δ-tocopherol. The oil with spices presented a similar behavior to that of the oil with the addition of TBHQ.  La concentración de tocoferoles en aceite de soja refinado (muestra control), aceite de soja adicionado de romero, orégano, ajo, semilla de achiote y TBHQ fueron cuantificados durante el almacenamiento durante 12 meses a 25°C y 35°C. El método propuesto para medir tocoferoles fue validado y determinada la incertidumbre. Este método presentó linealidad&#13; y precisión adecuadas, exactitud entre 93% y 103% además de una incertidumbre expandida de 2%. Las cantidades de α-, γ- y δ-tocoferol en el aceite de soja refinado, aceite de soja adicionado de condimentos y aceite de soja adicionado con TBHQ se mantuvieron constantes durante el almacenamiento a 25°C y 35°C con excepción del β-tocoferol el cual disminuyó. El aceite de soja adicionado de condimentos (romero, orégano, ajo, y semilla de achiote) presentó mayores concentraciones de γ- y δ-tocoferol en comparación con el aceite de soja refinado utilizado como control. El aceite de soja adicionado de condimentos presentó un comportamiento semejante al aceite de soja adicionado de TBHQ

Method for the validation and uncertainty estimation of tocopherol analysis applied to soybean oil with addition of spices and TBHQ