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Eight enzyme preparations were screened with a view to maximizing the yield of carrot seed essential oil. Three of the eight enzyme preparations investigated, lipase from Mucor circinelloides, XPect® pectinase, and Esperase® protease, significantly influenced the amount of essential oil obtained, with Esperase® being the most effective. The Taguchi method was applied to optimize the processing conditions for the Esperase® protease. Under the optimum conditions, the essential oil yield increased by approximately 48%. The main constituent compounds in the oil are: carotol (OeA: 40.80%–OeB: 46.17%), daucol (OeA: 7.35%–OeB: 6.22%), sabinene (OeA: 5.12%–OeB: 6.13%), alpha-pinene (OeA: 4.24%–OeB: 5.11%) and geranyl acetate (OeA: 4.50%–OeB: 3.68%). As compared to the control sample, the essential oil obtained from enzyme-pretreated carrot seeds has the same biological activity against Bacillus subtilis and Candida sp., lower activity against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa, and higher activity against Aspergillus niger and Penicillium expansum. Ocho preparados enzimáticos fueron seleccionados con el fin de maximizar el rendimiento de aceites esenciales de semillas de zanahoria. Tres de los ocho preparados de las enzimas investigadas, lipasa de Mucor circinelloides, Xpect® pectinasa y Esperase® proteasa, influyeron de manera significativa sobre la cantidad de aceite esencial obtenido, siendo Esperase® el más eficaz. El método de Taguchi se aplicó para optimizar las condiciones del procesamiento para esta última. Bajo las condiciones óptimas, el rendimiento de los aceite esenciales aumentó aproximadamente un 48%. Los principales compuestos constituyentes del aceite son: carotol (OEA: 40.80%–OeB: 46,17%), ducol (OEA: 7,35%–OeB: 6,22%), sabineno (OEA: 5,12%–OeB: 6,13%), alfa-pineno (OEA: 4,24%– OeB: 5,11%) y acetato de geranilo (OEA: 4,50%–OeB: 3,68%). En comparación con la muestra control, el aceite esencial obtenido a partir de las semillas de zanahoria mediante enzima-pretratada tiene la misma actividad biológica frente a Bacillus subtilis y Candida sp., menor actividad frente a Staphylococcus aureus, Escherichia coli, y Pseudomonas aeruginosa, y una mayor actividad contra Aspergillus niger y Penicillium expansum

grasas y aceites

The effect of commercial enzyme preparation-assisted maceration on the yield, quality, and bioactivity of essential oil from waste carrot seeds (&lt;em&gt;Daucus carota&lt;/em&gt; L.)