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Leukocyte response to mite (Dermatophagoides farinae) and mouse monoclonal anti-human IgE antibodies were assessed in 15 control subjects and 36 patients with nasal allergy to housedust and mite by means of in vitro histamine release assay from their leukocytes. The group of the thirtysix patients was consist of non-treated 14 patients and 22 patients who had received specific immunotherapy with housedust and/or mite for an average period 2.88 years. Levels of serum IgG and IgG4 antibodies specific to mite were estimated by an indirect enzyme-linked immunosorbent assay in the patients and the control subjects. The results were as follows: 1. No histamine release from leukocytes by mite was seen in 15 controls. The percentage of histamine release from leukocytes by mite was lower in treated patients than in non-treated patients (P=0.0314). 2. The levels of serum IgG4 antibody specific to mite were higher in treated patients than in non -treated patients (P=0.0253) . From this study, it may be concluded that the basophile reactivity to mite decreases with specific immunotherapy.

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Investigation of specific immunotherapy in patients with nasal allergy to housedust and mite: Histamine release assay from their peripheral leukocytes.