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Cholesteatoma matrix obtained from patients undergoing surgery for cholesteatomatous otitis media was cultured in Eagle MEM media containing of penicillin, streptomycin and gentamycin at 37°C, 5% CO2 in a moist environment. Skin lining the external auditory canal from the patients was also cultured in the same manner. Polyhedral, flat epithelial cells and fibroblasts usually outgrew within 2-3 days, and there was no significant difference of mode of outgrowth of both types of cultured cells. However, most of the outgrowth of the cells from cholesteatoma matrix slightly delayed. On electron microscopic observations of the polyhedral epithelial cells obtained from both cholesteatoma matrix and canal skin, there was no remarkable difference of the morphologic characteristics between them. The number of nucleolus, however, increased in most of the epithelial cells from cholesteatoma matrix. This findings may suggest of a neoplastic tendency of cholesteatoma matrix. Langerhans cell, melanocyte, Merkel cell and membrane-coating granules were not found in the submicroscopic investigations. The epithelial cells from cholesteatoma matrix as well as canal skin contained a few of keratohyalin granules and a lot of lipid granules. The overall morphology of the tissue culture cells was identical to that of the basal cells or spinous cells in the normal epidermis.

TISSUE CULTURE OF CHOLESTEATOMA MATRIX AND CANAL SKIN, AND ELECTRON MICROSCOPIC FINDINGS OF THE CULTURE CELLS