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Quality control of cryopreserved cord blood has assumed greater importance as the number of unrelated donor umbilical cord blood transplantations has increased. At present, we examine cryopreserved samples before cord blood transplantations, but it is unknown whether their results adequately reflect the quality of cord blood units. In this study, we tested the viability and recovery of colony forming cells (CFC) of 30 cord blood units (CB) and 2 kinds of samples. As test samples, we used segments (Seg) and tubes (Tube). With regard to viability, there was a high correlation between CB and sample type (CB vs Seg: r=0.938, CBvsTube: r= 0.939). Recovery of CFC of CB also correlated with sample type (CB vs Seg: r=0.879, CB vs Tube: r=0.817). These results suggested that the quality of CB could be determined by the testing of samples. Based on these results, criteria for cord blood transportation can be established.

QUALITY ASSURANCE OF CRYOPRESERVED CORD BLOOD UNITS BY EXAMINATION OF TEST SAMPLES