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To determine the optimal condition of blood samples applied to the stem cell monitor program of the automated hemotology analyzer, SE-9000, we examined the effects of anticoagulants, temperature and duration of storage regarding samples on immature information (IMI) and hematopoietic progenitor cell (HPC) counts, which were obtained from the IMI scatter gram of the analyzer.IMI and HPC cell counts were stable when EDTA-2K was used as an anticoagulant for blood sampling and the samples were kept at room temperature which was approximately 37°C. However, IMI and HPC cell counts should be measured within 4 hours after blood sampling, ever in optimal conditions. Concerning those samples kept in the conditions as described above, reproducibility of IMI and HPC cell counts was good in general, although coefficients of variation were higher at low count levels of HPC cells (HPC cell<40/μl). IMI and HPC cell counts significantly correlated with CD34-positive cell count in peripheral blood (r=0.738 and 0.798, respectively). In addition, HPC cell count in the apheresis products correlated with CD34-positive cell count and CFU-GM count in the products (r=0.620 and r=0.691, respectively). These findings indicate that IMI and HPC cell counts function as a predictor useful to determine the optimal harvest time of hematopoietic progenitor cells in peripheral blood, when the samples are maintained in an appropriate condition until counting can be performed.

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Evaluation of the optimal condition of blood sampling applied to the IMI (Immature Information) scattergram of the automated hematology analyzer SE-9000.