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The ability of leukocyte removal filters was investigated with three types of concentrated red blood cells (CRC); i.e., CRC prepared by a standard method (N-CRC), CRC suspended in Map (MapCRC) and buffy coat depleted CRC (BD-CRC). Pall RC50 filters with the same lots were used, and the same procedures were done in filtration to avoid the variations in filters as much as possible. Two units of each CRC stored for 1, 6 and 10 days was filtered through Pall RC50 filters. The mean residual leukocyte counts (n=12), which were calculated from the sum of each CRC stored for 1, 6 and 10 days, were as follows. For the 1st unit of N-CRC, Map-CRC and BD-CRC were 2.66 + 1.28 (X105), 1.55 + 0.25 (X105) and 1.44 + 0.25 (X105), respectively. For the 2nd units of BD-CRC, the mean count was 1.84 + 0.91(X 105), and 1.42 + 1.72 (X 106) for Map-CRC, while it was 6.58 + 105 to 1.38 X 108 [2.28 + 4.02 (X 107)] for N-CRC, showing much variation. The filter was obstructed with much microaggregate in one N-CRC sample stored for 10 days. The residual leukocyte count after filtration with this filter was high (8.16X107). These data suggest that the individual differences in the donor have a strong influence on the ability of leukocyte removal in N-CRC. BD-CRC and Map-CRC are more useful than N-CRC to bring out the ability of a leukocyte removal filter.

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Difference of leukocyte removal efficiency from three types of concentrated red blood cells (CRC) (normal CRC, Map-CRC and buffy coat depleted CRC)through one type of leukocyte removal filter.