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COMPARISON OF THE SENSITIVITY OF ABC-MACE, MACE, MAIPA AND FCM FOR THE DETECTION OF ANTI-PLATELET ALLOANTIBODIES Satoru Kosugi, Yoshiaki Tomiyama, Hirokazu Kashiwagi, Yoshio Kanayama, Yoshiyuki Kurata1) and Yuji Matsuzawa The Second Department of Internal Medicine, Osaka University Medical School 1) Department of Blood Transfusion, Osaka University Hospital Recently, antigen-specific assays for detecting anti-platelet alloantibodies have been available: immunobead assay, modified antigen capture ELISA (MACE) and monoclonal antibody-specific immobilization of platelet antigens (MAIPA). These assays are based on essentially the same principle. In this study, using anti-HPA-la and anti-HPA-4a antibody, we have compared the sensitivity of MACE using avidin-biotin-complex method (ABC-MACE), MACE, MAIPA, and flowcytometry (FCM) as a conventional method. The sensitivity of MACE for detecting anti-HPA-la antibody was twofold better than that of MAIPA, while the sensitivity of MAIPA for detecting anti-HPA-4a antibody was twofold better than that of MACE. Using ABC-method, the sensitivity of MACE for detecting antiHPA-la and anti-HPA-4a increased 8-fold and 64-fold, respectively. In comparison with FCM, ABCMACE showed almost the same sensitivity for detecting anti HPA-4a antibody, and showed better sensitivity for detecting anti HPA-la antibody. These data demonstrate that ABC-MACE is much more sensitive than MACE or MAIPA for detecting anti-platelet alloantibodies, and that the sensitivity of ABC-MACE is comparable to that of FCM.

Comparison of the sensitivity of ABC MACE, MACE, MAIPA and FCM for the detection of anti-platelet alloantibodies.