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The proposita was a healthy 51-year-old female donor who was brought to our attention when her serum reacted with all the cells tested except two Ko samples by saline, bromelin, albumin and anti-globulin methods.The proposita's cells failed to react with anti-K, anti-k, anti-Kpa, anti-Kpb, anti-Kpc, anti-Jsa, anti-Jsb and anti-Ku as expected. These negative reactions were reconfirmed by absorption-elution tests with appropriate sera. These findings indicated that the proposita was Ko with anti-Ku in her serum. Furthermore, she was found to be SS besides Ko, which is exceedingly rare in Japanese.A family study disclosed that the Ko phenotype occurred in two successive generations.Our experiments showed that the three samples of anti-Ku including the proposita's were absorbed by cells from the platelets layer, but the other antibodies of the Kell system such as anti-k, anti-Kpb, anti-Kpc and anti-Jsb were not.

nihon yuketsu gakkai zasshi/nippon yuketsu gakkai zasshi

CO-OCCURRENCE OF RARE PHENOTYPES Ko AND SS IN A JAPANESE DONOR WITH ANTI-KU IN THE SERUM AND A SUCCESSFUL ABSORPTION EXPERIMENT OF ANTI-KU WITH PLATELETS